Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements.
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PCR and serological tests have associated extremely fastidious organism with endometritis, PID, and tubal factor infertility.
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These media may require supplemental nutrients to obtain enhanced growth of nutritionally fastidious organisms such as pneumococci, streptococci, Abiotrophia, Haemophilus, gonococci, meningococci and Campylobacter.
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If one expects or looks for a particularly fastidious organism, the microbiological culture and isolation techniques will have to be geared towards that microbe.
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Many organisms responsible for endocarditis are slow-growing and may not be easily identified on routine blood cultures ( these fastidious organisms require special culture media to grow ).
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The predefined Etest gradient remains stable for at least 18 to 24 hours; that is, a period that covers the critical times of many species of fastidious and non-fastidious organisms.
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In 1991 he joined forces with a colleague, Dr . George Weinstock, and the two biologists decided the growth requirements of the fastidious organism could only be discovered by sequencing its genome, a feat impossible by the methods then available.
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Etest applications include many groups of fastidious organisms, fungi ( yeast and mould ) and mycobacteria as well as detecting various mechanisms of resistance and MIC testing of key antibiotics with critical specimens e . g . blood and cerebral spinal fluid ( CSF ).
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